Lengthening the number of cationic residues in a homopolymer from 6 to 12 in each branch reduced transfection using LPDs, most likely due to increased DNA compaction hindering the release of pDNA within the target cell. Mixed His/Arg cationic peptides were of particular interest, as these sequences have not been previously used in LPD formulations. The current study investigates the effects of novel Arg-containing cationic peptide sequences on the biophysical and transfection properties of LPDs. All peptides contained a linker sequence (cleaved by endosomal furin) attached to a targeting sequence selected to bind human airway epithelial cells and mediate gene delivery. Here LPDs have been prepared from cationic vesicles (composed of a 1 : 1 molar ratio of DOTMA with the neutral helper lipid, DOPE), singly branched cationic peptides and plasmid DNA. He received his Masters degree in Social Organization Psychology from Columbia and is currently an advisor and coach (TAC) Officer to the cadets at West Point.Lipopolyplexes (LPDs) are of considerable interest for use as gene delivery vehicles. He is jumpmaster qualified, attended Pathfinder School, SERE (Survival, Evasion, Resistance and Escape) school, and is a senior rated Aviator. His decorations include Bronze Star, Meritorious Service Medal, three Air Medals and the Combat Action Badge. He was deployed to Afghanistan and Iraq in support of Operations Enduring Freedom and Iraqi Freedom. Mark was selected to the Army’s Aviation branch and was trained to fly the UH-60 Blackhawk helicopter. Upon graduation he was commissioned as a Second Lieutenant through the Army ROTC program. Mark graduated from Colorado State University with a degree in Business Management. His most significant award is the Gordon Prize. He was involved in nearly every extracurricular activity SJMS offered. Mark graduated in 1993, the 105th Corps of Cadets and was the Battalion Executive Officer his senior year. He served on the Board of Directors Tim and his wife Suzan reside in Castle Rock, CO. In the 1980’s his family became part owner in a local bank, Community First. He is currently President and a member of the Board of Directors for the Soule Family Foundation. He was Chairman of the Board of Republic National Bank from 1987 to 1993. A member of the Board of Trustees for the joint NECA/IBEW Line Construction Benefit Fund. He is a member of the Board of Trustees of St. Tim was a Founding Member of the Board of Directors of Quanta Services from 1998 to 2000. a publicly traded NYSE company founded in 1998. Union Power Construction Company was a Founding Member of Quanta Services, Inc. The company installs transmission lines and sub stations over the mountains. Tim joined Union Power Construction Company in 1972 which his family owned and became Vice President in 1975.

Tim joined the Army for two years and served in Germany. After graduation, Tim enrolled and graduated from Westridge State College in Colorado. In 13 years he has been personally responsible for the saving of nearly 1,000 animals lives and gives away his considerable services as an expert canine trainer and behaviorist to any who ask for it.Ĭhuck embodies the spirit of the SJMS Old Boy thru his dedication and passion. He founded the Doberman Rescue in Colorado and has since lead a life in public service and volunteerism. He sustained a 4.0 gpa, wrote for the school newspaper and led the fight to make the university accessible and inviting to the handicapped. From there he moved back to his home in Colorado and enrolled at the University of Denver. He attended the University of SW Louisiana and sustained a 3.75 gpa, was a columnist for the school newspaper and was inducted into the Pi Eta Sigma National Honor Society. Chuck was permanently blinded in the line of duty.Ĭhuck attended the Veteran’s Administration Blind Rehab Center in order to learn as much as he could about succeeding in a new world. He competed in football basketball and rifle team, flag detail, color guard, honor guard, cadet patrol and a six time Blue Beret.Īfter graduation, he enlisted in the U S Army and was trained as a combat medic and operating room specialist. Chuck graduated from SJMS in 1985 as a Cadet Major with honors.

Salina journal